Sentence examples for by overlapping the sequences from inspiring English sources

The full length of the cDNA of this gene was obtained by overlapping the sequences of 3′- and 5′-extremities and amplification by reverse transcription-PCR.

The full length cDNA of this gene was obtained by overlapping the sequences of 3′- and 5′-extremities and amplification by reverse transcription PCR.

The full length of cDNA from this gene was obtained by overlapping the sequences of 3′- and 5′-extremities and amplification by reverse transcription PCR.

Full length cDNA was compiled by overlapping the sequences of PsEHa and RACE PCR products.

The nearly full-length genome sequence was assembled by overlapping the sequences of the two or three amplicons and merging them into one sequence as long as the two were greater than 98% homologous.

Contig sequences were obtained by overlapping the sequence information.

Amp-resistance cassette was amplified from pBS vector using primer 5' flanked by recombination arm overlapping the sequence upstream 87 region and using 3' primer flanked by recombination arm overlapping sequence downstream 87 region.

By overlapping the two partial cDNA sequences obtained by 3′ and 5′ RACE, their full-length cDNA sequences were obtained.

We gather structural neighbors from the VAST database that satisfy the default significance cutoff of P-value < 0.0001, such that folds are described by a continuous sequence region that overlaps the sequence fragment in the domain model by 90%.

One of these sites is located within the MGMT promoter (Table 1 and Figure 5A) and its Illumina probe overlaps the sequence tested by the PyroMark Q96 CpG MGMT kit used to validate our data (Additional file 7).

Complete sequences were reconstructed by overlapping shorter sequences, allowing us to verify that nucleotide changes were not attributable to RT-PCR artefacts.