Sentence examples for by overlap from inspiring English sources

splicing by overlap extension PCR.

Target genes were amplified by overlap PCR.

The new method is called protein domain library generation by overlap extension (PDLGO).

The three purified promoter, coding sequence and terminator fragments were assembled by overlap extension PCR (OE-PCR).

These fused genes were generated by overlap extension PCR, and the linker sequence between them was 5′-CTGGACAGCACC-3′.

The amplified dkr fragment was fused to the promoters P sppA and P sppQ by overlap extension polymerase chain reaction.

Sequence reads can be assembled either by anchoring them directly onto an available reference genome (classical reference assembly), or can be concatenated by overlap (de novo assembly).

Precise, in-frame deletions in sipF, sfp2, sfp1 and srtF were constructed by using splicing-by-overlap-extension PCR [48].

DNA-fusion constructs were generated by overlap-extension PCR.

Then they were ligated successively by overlap-PCR and finally inserted into T-easy vector.

Mature boophilin (variant H2) was cloned into the bi-cistronic expression vector pRBI-DsbC [63] by overlap-extension PCR.