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The GCIP produced by our optimized method(13) consistently produced ∼115 μA of C− and accurate and precise Fm value with a throughput of up to 270 AMS targets per skilled analyst/d for biological/biomedical AMS applications.
Formation of graphite in 3 h at 500 °C by our optimized method was consistent with prior reports that graphite formation can be >95% completed in 1 h at 600 °C 35) to 4 h at 500 °C.
AMS targets were prepared by our previous method(7) as well as by our optimized method(13) for which we built a new heating block with 7 rows, 14 holes apiece, to perform at 525 °C ± 0.3 (hole to hole).
The δC of 1-mg aliquots of solid C was −18.4 ± 0.2‰ by our previous method(15) and was −17.9 ± 0.3‰ by our optimized method, and the δC especially by the optimized method was within the accepted range (−17.8 ± 0.05‰).
After reduction by our optimized method, 13) the Zn dust was deformed and appeared as a thinner (compressed) and more fibrous Zn band at 240 °C, a Zn mirror at 380 °C, and a softer Zn cake with less sinter at 500 °C that stuck to the bottom of the septa-sealed vial.
It also summarized the physical and morphological characteristics of AMS targets of iron-carbon materials (ICM) (produced 20% of the time) and GCI (produced 80% of the time) by our previous method(7) and of GCIP (produced 100% of the time) by our optimized method.
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Table 2 compared the Fm values of four C sources as measured by our previous(15) and our optimized method in the present paper.
The limits of quantitation were found to be 35 and 4 ng ml−1 for Pb and Cd, respectively, by the optimized method.
Xylanase was assayed by the optimized method described by Bailey et al. [22], using 1% birchwood xylan as the substrate and xylose as the standard.
In contrast, a smaller box was constructed by the optimized method, which resulted in the final conformation of NADP that has an RMSD of 2.7 Å. Thus, using the optimized search space significantly improved the accuracy of NADP binding pose prediction by Vina.
Within a distance of 1 Km, for instance, the maximum clinic accessible for 1,000 persons in one DA, when it is estimated by the conventional method is approximately 2.404, compared with 27.706 physicians for 1,000 potential users when it is estimated by the optimized method.
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