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More complete validations of antisense transcripts detected by our microarray platform were conducted by RT-PCR, Northern analysis, and in situ hybridization in our previous work [ 11].
Moreover, previously known antisense transcripts, such as those arising from Myc (myelocytomatosis oncogene) [ 22] and Tgfb2 (transforming growth factor beta2) [ 23], were also detected by our microarray platform (data not shown).
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To summarize, the overall trends of temporal changes of expression and binding by SMAD4 observed in our microarray platforms were recapitulated by the RT-PCR and ChIP-PCR experiments, respectively.
Consequently, the fact that we found these genes regulated underlines that the regulation data retrieved by our DNA microarray platform are biologically relevant.
The client application allows user to query TBrowser database using six methods: by gene symbols, by probe IDs, by experiments, by microarray platform, by ontology terms (annotation) or by TS.
On this basis, to identify cellular genes that are modulated by HCV RNA replication, we analyzed the expression profile of cell lines carrying a full-length HCV genome by a new microarray platform developed by the Applied Biosystems.
BPDCN microarray data of 6 cryopreserved tissue samples (Sapienza et al., 2014) by Affymetrix microarray platform were normalized with the rank invariant method using Lumi (Bioconductor) (Du et al., 2008).
The next level of clustering was defined by the microarray platform used for the studies.
Overall over 90 percent of the miRNA microarray results tested were validated by qPCR, confirming the integrity of the data generated by the microarray platform.
Probes and hybridizations were performed by the Microarray platform of the IGBMC and Génopole Alsace-Lorraine (http://www-microarrays.u-strasbg.fr).fr
The gene expression profiling from 562 ovarian patients was measured by UNC_AgilentG4502A_07_3 microarray platform.
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