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Previous studies suggested a primitive fingerprint of murine and human iPSC-derived macrophages30,36,37, which is further supported by our microarray analysis.
The starch content was higher in both roots and shoots grown under Zn-deficient conditions (Figure 1a), corresponding to the gene expression pattern revealed by our microarray analysis.
Six miRNAs showed similar expression patterns as those revealed by our microarray analysis.
The fold differential expression rate also did not differ significantly among tumors of low or high grade, as acquired by our microarray analysis.
The fold expression (mean±SD) of the GOIs in each one of the three tumor groups compared to the normal tissue, as acquired by our microarray analysis, is depicted in (Figure S2).
The most significant gene network (p = 10−50) consisted mainly of proteins that were up-regulated in active patients (by our microarray analysis); AKT, NF-κB, HSP90, proteosome, IER3 and HSPB1 emerged as central nodes (Figure 2C).
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Furthermore, we have previously shown that LhrA acts to destabilize the lmo0850 mRNA [23], but to our surprise, lmo0850 was not identified as being affected by LhrA in our microarray analysis.
Consistent with our previous observations in LB, we found four small non-coding regulatory RNA genes (THI, csrB, rnpB, tke1) and three mRNA transcripts (rpoS, sufE, fliC) regulated by Hfq in our microarray analysis in M9 media (7 of 38 or 18%).
Among the genes upregulated by TOX3 in our microarray analysis was the well-studied pro-metastatic gene CXCR4 [ 26- 28].
Because D-Mef2 mutation suppresses PAX7-FOXO1 anD-Mef2f2 is overexpressed by PAX7-FOXO1 in our microarray analysis, we investigated whether D-Mef2 acts as a downstream PAX-FOXO1 target.
We further tested the validity of our microarray analysis by determining by qPCR the expression of a miR19b, a microRNA known to be down-regulated in normal aging (i.e. octogenarians)9.
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