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The mRNA expression for 12 hES-MSC samples was profiled by our lab using the Illumina Ref8 v3 Expression BeadChip (data not shown).
Prior investigations by our lab using calcium ionophore, glucocorticoid, and thapsigargin have successfully identified several biophysical changes that occur in the cellular membrane during apoptosis [ 3, 4, 7– 9].
Additional primers were designed in Primer3 based on flanking sequences from microsatellite repeats identified from the Eukaryotic Microsatellite Database (http://www.veenuash.info/) or by our lab using the Col-0 reference genome sequence (Arabidopsis Genome Initiative 2000).
The tumour size, 160±28 mm for placebo tumours and 228±60 mm E2-treated tumours, was in the range previously described by our lab using B7-1/IL-2 and other cytokines delivered to PyMT tumours by adenoviral vectors (Addison et al, 1998; Emtage et al, 1998; Palmer et al, 2001).
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This is supported by a separate study in our lab using N-acetyl cycteine (NAC) as the inhibitor for ROS to confirmed that the action of GTT was through the induction of H2O2 (unpublished data).
Samples were genotyped using either the Sequenom™ system (Sequenom™ Autoflex Mass Spectrometer and Samsung 24 pin nanodispenser) by the Australian Genome Research Facility (www.agrf.com.au) or by our university lab using Taqman® SNP genotyping assays (Applied Biosystems, USA) utilising minor groove-binder probes.
HPLC method was established by our lab and was used for quantitative determination of STS.
The novel expression vector pHsh, constructed by our lab [19] [20], was used for expression of glmS in E. coli.
The V. volvacea cDNA library, constructed by our lab previously, was used as the template for amplifying the gfat cDNA fragment.
However, our finding is consistent with a recent study by the Dantuma lab using a transgenic mouse expressing a different UPS reporter substrate [24].
In our study, however, all the LCLs came from a single biobank (NLGIP) and were generated by a single lab using the same stock of B-95 EBV-expressing marmoset cell line [ 25].
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