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This observation was confirmed by our SplitsTree analysis.
This interpretation is supported by our SplitsTree analysis (see Additional File 1) and the present molecular results (see Figure 1), which clearly signal independent evolutionary origins for all the different mesopsammic Heterobranchia included here.
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The above observations were confirmed by the Splitstree analysis (Figure 7A).
The recognition of major gene pools or evolutionary lineages is best illustrated by the SplitsTree analysis based on the ITS.
The presence of an ascus is currently accepted as a unique synapomorphy of Sacoglossa [ 4], and any sacoglossan relationship is clearly rejected by SplitsTree analysis (see Additional file 1) and phylogenetic analyses in the present study.
This network reconstruction could be achieved by different means, either through the recoding of the PAMs features in a matrix of characters or by realizing a Splitstree analysis (for instance) of the whole alignments of Ty3/Gypsy and Retroviridae sequences.
The Blosum62 matrix was used as the distance measure in the Splitstree analysis.
SplitsTree analysis yielded a network, with some reticulations but also with long branches (Fig. 2).
The first of them was SplitsTree analysis which was performed on the base of allelic profile data of Bcc STs.
Minor allele frequencies for all 125 SNPs reported here are listed in Additional file 2. The SplitsTree analysis produced a NeighborNet phylogenetic network that separates the cutthroat trout subspecies.
This inference was further supported by our multivariate analysis.
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