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By optimizing the separation with gradients of increasing complexity, acceptable resolution was possible, being glycine/threonine the critical pair.
Mature VEGF-D and the glycosylation mutant were each enriched for the biologically active dimeric form by optimizing the separation of dimer from monomer via gel filtration, followed by conversion of remaining monomers to dimers via treatment with cysteine.
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Spectroelectrochemical measurements have been carried out with the system Fe(bpy)2+3 + 0.1 M KCl/Ag by using laboratory-built equipment optimizing the separation from the noise and collection of the Raman signals to study adsorption of the complex of 2,2-bipyridil with Fe2+ on Ag.
Potential co-elution of 2-NP isomers with 4-NP1EO was considered when optimizing the separation.
This information can be very useful in optimizing the separation of a mixture of known compounds.
An improvement of the quality of separation might be possible by optimizing the dimensionality reduction, but the effort was not undertaken here.
Once the three-dimensional strong swirling flow is fully understood, cyclone performance such as pressure drop and separation efficiency can be improved by optimizing the cyclone design.
At present work, the relatively uniform shell layers were attained by optimizing the working pressure and power, which was beneficial for light absorption and carrier separation.
Further separation based on the size and the shape of the glycans needs a more careful optimization of the BGE, but can be achieved by optimizing the pH and the concentration of organic modifiers in the BGE [ 104, 105].
This problem was solved by optimizing the synthesis of ZnO.
These parameters have been estimated by optimizing the prediction process.
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