By optimizing the multiplex PCR conditions, where concentrations of buffer, dNTPs, and primer pairs were major factors, satisfactory sensitivity and reproducibility of the band patterning were achieved.
We have developed and optimized a high multiplex PCR amplicon sequencing process, which can accommodate hundreds of target specific primers containing molecular barcodes in a single reaction.
The purpose of this study was to develop and optimize a DPO multiplex PCR assay for detection of influenza A and B viruses, including the influenza A subtypes, such as seasonal H1N1, 2009 pandemic H1N1, H3N2, and avian influenza H5N1.
Primers and probes specific for HSV and VZV genes were combined and optimized in a multiplex PCR.
The multiplex PCR reaction system was established by optimizing the reaction condition, and a series of tests on the sensitivity, specificity, and clinical application were also conducted.
In this sense, we have optimized a single-cell multiplex PCR method to simultaneously amplify a wide spectrum of STRs within the HLA and β- globin loci, which minimizes the preclinical development time for the families.
We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1.
Consequently, these assays have to cope with several issues: identifying informative markers near the mutation; optimizing the multiplex format of the single-cell PCRs to minimize ADO; and avoiding misdiagnosis resulting from a possible disconnection of linkage between the mutation and a linked polymorphic marker allele caused by an intervening homologous recombination.
Numerous respiratory pathogens can be detected from a single sample by using a multiplex molecular method called target-enriched multiplex PCR (3– 6).
Samples also underwent NAT for other respiratory viruses by using a multiplex PCR.
