Pro-CLARANS randomly selects one of C neighbors, C*, which differs by only one sequence.
Putative SNPs identified from such contigs would have the minor alleles represented by only one sequence.
We did not find sequences belonging to rare haplogroup X2a in our sample, whereas the other rare haplogroup D4h3 is represented by only one sequence.
We also noticed another small inversion that was supported by only one sequence read and caused SNPs at position 18, 20, 21 and 23 (rpsbA- psbA IGS).
The mean coverage of each contig is 2.4 with less than half of the contigs represented by only one sequence read.
Of my 10 bacterial phyla one (Chlorobacteria) is represented by only one sequence and five are clearly monophyletic (Archaebacteria, Spirochaetes, Hadobacteria, Cyanobacteria with strong support, and Sphingobacteria with moderate (40 80%) support).
This incongruence may have arisen because except for a few predominant superfamilies, 19 and 16 conopeptide groups in C. tribblei and C. lenavati, respectively, were each represented by only one sequence.
Two different data sets were analyzed: (1) the SSU alignment of 33 Zygnematophyceaen taxa (including 12 Spirogyra sequences representing the different clades) and (2) the SSU alignment of 55 Spirogyra sequences (clones with identical sequences were represented by only one sequence).
Because they were not informative and created aberrant multi alignments during the re-samplings procedure, a total of 47 sites were removed from the alignment (12 of them were represented by only one sequence and the 35 others were non or few-informative sites, among them 29 were only represented by the three type VII wheat nsLTPs).
MITF is a transcription factor involved in melanocyte differentiation and survival and even though it is represented by only one sequence in the Met library, its differential expression between the RGP WM1552C and the metastatic cell lines was confirmed by Northern blot (Fig. 3E).
