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Notably, the Northern Group was separated by one mutational step from the central haplogroup, while the southern haplogroup was separated from the northern haplogroup by one mutational step.
For this reason, the infinite allele model was used in which any allelic state can be reached by one mutational step.
C2.R.U.0701 and C74.R.U.0800 differed by one mutational step from the most common genotype, and C10.R.U.0701 differed by one nucleotide substitution from C2.R.U.0701.
A less conservative estimate, based on counts of all colonies with more than one distinct genotype within a colony, including those that displayed just one non-shared allele (excluding single allele difference by one mutational step), indicates that 5% of colonies in both populations were chimeras.
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The haplotype network (Fig. 2b) revealed that most NE haplotypes are connected to each other (generally differing by only one mutational step) in a star-like fashion with a central haplotype (Mlh16), which is also found in SE populations.
C11.S and C27.S were single locus variants of each other, consistent with the close evolutionary relationship (one mutational step) found by the statistical parsimony procedure.
The analysis included only pairs whose codons were one mutational step apart.
With the exception of one shared haplotype, one mutational step separated the two groups of water rails at PTPN12.
The haplotype relationships of M. fascicularis were reconstructed by assuming that at any given site, two randomly drawn haplotypes were unlikely to have arisen from more than one mutational step [ 42].
The size of the circles corresponds to the frequency of each haplotype and each solid line represents one mutational step.
At the other end of the network, there was an isolated node (R1) connecting twenty-five animals within one mutational step.
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