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Controls were performed by omitting the reverse transcriptase enzyme.
Negative controls were performed by omitting the reverse transcriptase (not shown).
All templates were checked for DNA contamination by omitting the reverse transcriptase in control reactions.
RNA samples were controlled for genomic contamination by omitting the reverse transcriptase in the cDNA synthesis mixture.
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Minus reverse transcriptase (NoRT) control reactions were prepared for each sample by omitting the Stratascript™ reverse transcriptase.
Furthermore, possible contamination with genomic DNA was excluded by control experiments omitting the reverse transcriptase.
The possible contamination with genomic DNA was excluded by control experiments omitting the reverse transcriptase.
Controls for DNA contamination were performed omitting the reverse transcriptase.
Controls for DNA contamination were performed omitting the reverse transcriptase during reverse transcription.
Control RT reactions omitting the reverse transcriptase were included for each sample.
No PCR amplification was revealed when omitting the reverse transcription step (data not shown).
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