Results are reported in Figure 2. The fold differences determined by qRT-PCR were very similar to those obtained by the high density oligonucleotide microarray gene expression analysis.
Oligonucleotide microarray gene expression data.
By means of oligonucleotide microarrays, gene expression profiles of salivary glands at 4, 8, 12, 16, and 20 weeks of age were generated for C57BL/6.NOD- Aec1Aec2 male mice.
Altogether, these results confirmed that rsh-dependent differential gene expression evidenced by oligonucleotide microarray analysis could be reproduced by RT-qPCR.
Finally, the 57,307 sequences were integrated into 43,236 unique sequences, i.e. the 43k EST assembly, for use in gene space enrichment by oligonucleotide microarray.
In the present study, we examined gene expression in the gastric mucosa in a H. pylori-infected and high-salt diet-treated mouse gastric tumor model by oligonucleotide microarray and found two candidate up-regulated genes including Cd177 and Reg3g.
Estilo CL, O-charoenrat P, Talbot S, Socci ND, Carlson DL, Ghossein R et al. Oral tongue cancer gene expression profiling: Identification of novel potential prognosticators by oligonucleotide microarray analysis.
Thimmulappa RK, Mai KH, Srisuma S et al. "Identification of Nrf2-regulated genes induced by the chemopreventive agent sulforaphane by oligonucleotide microarray".
Genome wide expression analysis by oligonucleotide microarray is a powerful molecular tool for the discovery of new genes involved in molecular processes including drug resistance.
This deletion was confirmed by oligonucleotide microarray (targeted 60K oligonucleotide microarray performed at GeneDx, Gaithersburg, MD), as well as quantitative PCR of one of the genes (TAF4) in the deleted interval.
