Sentence examples for by oligodT priming using from inspiring English sources

Up to 2 ug of mRNA were then reverse transcribed for 90 min at 37 °C by oligodT priming using the Omniscript® reverse transcription kit (Qiagen, Hilden, Germany).

cDNAs were synthesized by random priming, using purified total RNA as template.

The lacO probe was labeled by random priming, using as template a ∼300 bp fragment containing LacO repeats.

cDNA was subsequently synthesized from 5 μg RNA by random priming, using Superscript II (Invitrogen).

After DNase I digestion (Roche), 2 μg RNA was reverse-transcribed by random priming using Superscript II (Invitrogen).

The cDNA probes were labeled by random priming using 32 P-dATP (cDNA sequences available upon request).

RNA was purified from around 50 colonies using RNeasy Mini Kit (Qiagen) and cDNA subsequently generated by Oligo-dT priming using SuperScript First-Strand Synthesis System (Invitrogen).

RNA from whole liver (5 μg) or sorted cells (entire yield) was transcribed to cDNA using oligodT priming and Superscript III (Life Technologies).

Complementary DNA was produced from oligodT-primed mRNA using the Omniscript RT Kit (Qiagen).

A. viridis cDNA library was generated at the RZPD (Deutsches Ressourcenzentrum für Genomforschung GmbH, Berlin, Germany) by oligodT, random priming and directionally cloning into pSPORT1.

The pCI- SMN2cDNA rescue plasmid was constructed by reverse transcription of HEK-293T total cellular RNA with oligodT primers using Superscript III cDNA synthesis kit (Invitrogen, Carlsbad, CA).