Kwark et al. [28] recently confirmed this theory by optically observing a single circular nanoparticle coating formed on a boiling surface, where a single-active bubble nucleation site was allowed to undergo several boiling cycles, as shown in Figure 10.
By contrast, we observe a single peak for the insertion of Gypsy elements and Non-Autonomous LTR retrotransposons.
In support of this, we have analysed many hundreds of fibrinogen samples by ESI MS and never observed a single incidence of a circulatory fibrinogen molecule lacking both sialic residue.
We tested the efficiency of our protein pull-down by Western Blot, on which we observed a single band for GR-tagged RGL2.
Quality control of the pooled library was monitored by first observing a single band on an Agilent bioanalyzer using a High Sensitivity DNA Kit (5067 4627; Agilent) for double-stranded DNA and last by determining molecules per microliter using quantitative PCR techniques (KK4821; KAPA Biosystems).
In Experiment 1, variation in the courtship interactions of flies from mass-bred populations from Oulanka and Vancouver was traced among the four cross combinations (O×O, O×V, V×O and V×V; O = Oulanka, V = Vancouver, with females always listed first) by observing single mating pairs in individual plastic observation dishes for a maximum of two hours.
Albeit we are aware that one cannot reach general conclusions by observing one single instance, we are convinced that careful observation of one empirical reality can shed light on what sort of research questions should be asked.
This can be done by observing how far a single event, like a heat wave, is outside the norm of prior events, or by using computer models to determine how often an event like it would occur with or without human greenhouse gas emissions (which can be included or excluded from the model).
By observing whether the single fly quickly approached a mate and stayed put, they could measure its pickiness.
