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No discrepancies were found between the array typing and the genotype identity confirmed by nucleotide sequencing of the PCR product.
The PCR subtyping results matched completely with those obtained by nucleotide sequencing of the env V3 region.
In the present study, primary structures of these toxins were determined by Edman degradation and by nucleotide sequencing of the cDNA clones.
Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions.
This was further confirmed by nucleotide sequencing of the PCR amplified products (Figure 3D).
Mutation analysis of mutT2 and mutT4 was performed by nucleotide sequencing of these genes.
Similar(30)
RLK predicted by nucleotide sequences of rlk was a 549 amino acid -long type II keratin.
The non-coding regulatory region (NCRR) is hypervariable, as evidenced by nucleotide sequence of the non-virulent variant, which is predominantly excreted in urine, versus that of virulent variants found in brain and CSF of PML patients.
More recently, a sister group relationship between Cestoda and Trematoda was supported by nucleotide sequences of the combined 18S and 28S ribosomal genes (Lockyer et al. 2003), mitochondrial DNA (Park et al. 2007; Perkins et al. 2010), and microRNA loci (Fromm et al. 2013).
Furthermore, mutations were not detected in KRAS or PIK3CA (exon 9 and 20) in these four tumors by nucleotide sequencing analysis of the PCR products or clones of the PCR products (data not shown).
Primer specificity was confirmed by nucleotide sequencing (MWG, Germany) of amplicon.
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