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No multiple infections with different HHV8 variants were identified by nucleotide sequencing analysis.
Furthermore, mutations were not detected in KRAS or PIK3CA (exon 9 and 20) in these four tumors by nucleotide sequencing analysis of the PCR products or clones of the PCR products (data not shown).
Similar(58)
Furthermore, more than 20 bp distances was placed between the splicing site and 3′-end of at least one of a primer in each pair, which may be used to authenticate amplicons by nucleotide sequence analysis.
Pilot experiments on isolates from twenty different species showed that the designed primer sets successfully detected all species by RT-PCR, as confirmed by nucleotide sequence analysis of the amplicons.
The correct sequence of the generated plasmid was confirmed by nucleotide sequence analysis.
Clones with the insert in the forward orientation were identified by nucleotide sequence analysis.
Rif resistant clones were characterized by nucleotide sequence analysis of a 1663 bp fragment of rpoB that included the positions conferring Rif resistance in the donors (Fig. 1B).
We assumed an MAA biosynthesis route shown in Figure 2. Our EST matching approach by nucleotide sequence analysis or by KEGG BRITE protein classification did not yield directly any strong candidate genes likely involved in MAA biosynthesis.
The sequences were confirmed by nucleotide sequence analysis.
The integrity of each clone was verified by nucleotide sequence analysis.
The amplification product was tested by electrophoresis through a 1.0% agarose gel and by nucleotide sequence analysis.
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