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emm type was determined by nucleotide sequence typing as described [38], and emm type was assigned using the BLASTn-emm server (http://www.cdc.gov/ncidod/biotech/strep/strepblast.htm).htm
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Only 113 (55.4%) were corroborated by nucleotide sequence.
The sequences were confirmed by nucleotide sequence analysis.
Groups of genetically related meningococci can be identified by using the electrophoretic mobility of cytoplasmic proteins (electrophoretic type; ET) or by nucleotide sequencing of "housekeeping" genes (sequence type; ST).
Enteroviruses identified by pan-enterovirus RT-PCR were typed by nucleotide sequencing of the viral protein 1 (VP1) region (8, 9 ).
Isolated enteroviruses were typed by nucleotide sequencing of VP1 and VP4 genes and genogrouped by phylogenetic analysis [ 11, 12].
Each isolate was typed successfully by nucleotide sequencing of either partial ORF1a or ORF2 products obtained by using primers Mon340/348 (n = 5) and Mon244/245 (n = 1), respectively (Table).
RLK predicted by nucleotide sequences of rlk was a 549 amino acid -long type II keratin.
No discrepancies were found between the array typing and the genotype identity confirmed by nucleotide sequencing of the PCR product.
The typing of these agents is generally done by nucleotide sequencing, blot hybridization, or enzyme immunoassay.
The construct was analyzed by nucleotide sequencing and alignment with sequences of H. pylori strain 60190 (s1/ m1 allelic type) vacA sequence (GenBank accession number U05676), and strain 95-54 (s1/ m2 allelic type) (GenBank accession number U95971).
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