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Saccharomyces sensu stricto species are post-zygotically isolated due to a failure of inter-species homologous chromosomes to segregate properly in meiosis I, which is caused in part by nucleotide sequence divergence acted on by the mismatch repair machinery (Greig et al. 2003; Liti et al. 2006).
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Low Shimaa strain genetic diversity is further illustrated by median nucleotide sequence divergence per site (Fig. 2): 1.3% among Shimaa strains, vs. 1.8% among Japanese, and 3.2% and 3.9% in Spanish and urban Peruvian strains, respectively.
Compared to the Tabasco strain, the other Mexican isolates differed by 0.55%0.66%% nucleotide sequence divergence across the genome.
Isolates from immunodeficient VAPP (iVAPP) patients and some asymptomatic carriers show evidence of prolonged replication as indicated by >1% nucleotide sequence divergence from the corresponding Sabin OPV strain; such vaccine-derived polioviruses (VDPVs) isolated from immunodeficient persons after exposure to OPV are called iVDPVs (6, 7 ).
Within class 1, copies fall into four distinct groups (differing by more than 10% nucleotide sequence divergence, which we call copy types) in all four species.
Estimated nucleotide sequence divergence varies by chromosome and is lowest on chromosome X (fig. 2), a finding consistent with those of previous primate comparative genomic studies (e.g., The Chimpanzee Sequencing and Analysis Consortium 2005).
A forward stepwise multiple regression model was used to examine the influence of four potential explanatory variables (predictors) on asymmetry in nucleotide sequence divergence (as measured by nucleotide asymmetry/site) of paralogs for 130 duplicate pairs.
The extent of nucleotide sequence divergence was estimated by means of the Kimura 2-parameter method [ 63] and the amino acid sequence divergence estimated by means of the uncorrected differences (p-distances).
This has been confirmed by the highest RAPD genetic similarity and the lowest pairwise nucleotide sequence divergence between the two species as revealed in this study.
A simulation study by Vijay et al. [ 59] demonstrated that reference genomes with average nucleotide sequence divergence up to 15% can help improve transcriptome assemblies while with greater divergence there was no improvement over a de novo assembly.
Such a relatively high nucleotide sequence divergence is similar to those observed in reverse transcriptases encoded by non-LTR retrotransposons and in some DNA transposases.
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