Sentence examples for by nucleotide sequence analysis from inspiring English sources
Pilot experiments on isolates from twenty different species showed that the designed primer sets successfully detected all species by RT-PCR, as confirmed by nucleotide sequence analysis of the amplicons.
Furthermore, more than 20 bp distances was placed between the splicing site and 3′-end of at least one of a primer in each pair, which may be used to authenticate amplicons by nucleotide sequence analysis.
Clones with the insert in the forward orientation were identified by nucleotide sequence analysis.
The correct sequence of the generated plasmid was confirmed by nucleotide sequence analysis.
Rif resistant clones were characterized by nucleotide sequence analysis of a 1663 bp fragment of rpoB that included the positions conferring Rif resistance in the donors (Fig. 1B).
We assumed an MAA biosynthesis route shown in Figure 2. Our EST matching approach by nucleotide sequence analysis or by KEGG BRITE protein classification did not yield directly any strong candidate genes likely involved in MAA biosynthesis.
Furthermore, mutations were not detected in KRAS or PIK3CA (exon 9 and 20) in these four tumors by nucleotide sequencing analysis of the PCR products or clones of the PCR products (data not shown).
All cDNA inserts were validated by complete nucleotide sequence analysis.
Boundaries of the genomic islands encoding T6SS were defined by thorough nucleotide sequence analysis.
The genetic variability of virus isolates was further investigated by performing nucleotide sequence analysis of the VP1 capsid region of isolates that were identified as possible VDPVs by the VDPV screening assay.
