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The level of phosphorylation of Serine 1928 on Cav1.2 protein was determined by normalizing the obtained p1928 band to the total CNC1 intensity for the loaded surface fraction.
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DNA quality is assessed by normalizing the concentration obtained with the 129 and 305 bp amplicon against the one obtained with the 41 bp assay (Q-score).
EGD ratios were computed by normalizing the EGD obtained at the shortest S1-S2 interval (a − b) at which the S2 extra stimulus resulted in a BEG, with the EGD obtained at the longest S1-S2 interval (c − d) to give the expression (a − b)/(c − d).
Absolute counts were obtained by multiplying the number of events detected in each subpopulation of events by the volume of the suspension analysed by the cytometer and by normalizing the result obtained to 1 ml mouse blood (taking the dilution factor of the blood into account).
The EGD ratio was calculated by normalizing the EGD obtained at the shortest S1S2 interval to one following the S2 stimuli imposed at the longest S1S2 intervals in the stimulus cycle.
DNA quality is assessed by normalizing the concentration obtained with the 129 and 305 bp amplicon against the 41 bp amplicon (Q-ratio), where a Q-ratio of 1 represents intact DNA.
The percentages of interactions with Hsp60 were obtained by normalizing the emPAI values obtained for the Hsp60 interactors in each temperature conditions and cellular fractions by the sum of all emPAIs values for each condition.
Then we calculated symmetrical difference for the gene trees with artificial transfers and normalized the obtained values by the average value of symmetric difference of the original data minus two standard deviations.
Detection of copy-number alterations (CNAs) was performed by obtaining a log-ratio profile of the sample by normalizing the sequence coverage obtained at all exons against a process-matched normal control.
Current densities were determined by normalizing the current amplitude obtained at +80 mV 200 s after break in to the cell membrane capacitance.
Relative expression was calculated by normalizing the results by these obtained for non vernalized plants.
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