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The activity of FAK and Pyk2 was determined by normalizing the intensity of the pFAK Y397) and pPyk2 Y402) bands to total FAK or Pyk2 bands, respectively.

Ser235 phosphorylation was compared in the three different conditions by normalizing the intensity of the SVSERLpSILK phosphopeptide to that of the total ion chromatogram.

The expression levels of BAG2 are expressed in terms of a log2 median-centered intensity which is calculated by normalizing the intensity of BAG2 probe to the median of the probe intensities across the entire array.

In addition, violaxanthin, antheraxanthin, and zeaxanthin (VAZ) pool was calculated by normalizing the intensity (area) of signals detected by UV detector for violaxanthin, antheraxanthin, and zeaxanthin in total to zeaxanthin for which standard zeaxanthin was used for quantification.

Potential effects of technical variability were minimized by normalizing the intensity values to the total ion current (TIC) such that the summation of all feature intensities in each individual sample were equal.

Similar(55)

The grey-level transformation step starts by linearly normalizing the intensity between levels 0 and 1. Next, the pixels are assigned a grey level.

From these scans, we obtained a relative transmissionaTrel(red dashed line in Figure 1) by normalizing the measured intensity Im with the mean background intensity Ib from regions without particles: Trel=Im/Ib.

Bands were quantitated using reverse image scanning densitometry (Photoshop CS2, Adobe) by normalizing the band intensity of the co-immunoprecipitated protein to the band intensity of the immunoprecipitated protein.

The percentage relative fluorescence intensity of in G14-GAL4>VAPwt and G14-GAL4>VAPP58S was calculated by normalizing the control intensity to 100 percent.

After the NMR experiment, the relative signal intensities (integrated peak areas) of H NMR peaks at each oxygenation state were determined by normalizing the signal intensity to its full oxygenated and deoxygenated condition.

By normalizing the ion intensity of peptides with respect to the glass-microfiber matrix ([SiOH]+), a linear calibration curve for each peptide was generated to quantify these components in a mixture.

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