Sentence examples for by normalizing the gene from inspiring English sources
Relative gene expression was determined by normalizing the gene expression of each target gene to GAPDH.
The ΔΔ Ct values were calculated by normalizing the gene expression levels to β -actin.
Raw CN was determined by normalizing the gene dosage ratio of unknown samples to that of the calibrator.
The LD gene signature was created by normalizing the gene expression values in the +R5020 treatment group to the -R5020 treatment group, then comparing those normalized fold change values between the KR and WT PR expressing cell lines.
Data analyses were performed by normalizing the gene expression data using the lumi Bioconductor package in the statistical language R and by clustering relative gene expression to assess the expression profiles of the different samples.
The ligand-independent (LI) gene signature was created by normalizing the gene expression values in -R5020 treatment group in WT or KR expressing cells to the -R5020 treatment group in the PR-null expressing cells, then comparing those normalized fold change values between the KR and WT expressing cell lines.
In IntPath, we overcome the above two limitations by normalizing the pathway gene representations and gene relationship representations from different databases into unified IntPath gene and relationship representation.
The usefulness of 8 genes among them was tested by normalizing the relative gene expression against test genes whose expression pattern was known.
For statistical analysis, ΔCt expression values of each gene were obtained by normalizing the raw gene values to 18S rRNA as a reference gene.
Relative expression levels for each gene of interest were calculated by normalizing the target gene transcript level (Ct) to the respective GAPDH level as described previously (2−ΔΔCt formula, Perkin Elmer User Bulletin #2).
Mitochondrial DNA was quantified by normalizing the mitochondrial-encoded gene COXI (mtco1) to the nuclear-encoded gene Ndufv1 using qPCR and the ∆∆CT method.
