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To compare our mice expression profiles with those of human gastric cancers, the gene signature to classify human intestinal- and diffuse-type gastric cancers was also modified from original one by normalizing the expression data to the average of normal gastric samples.
Samples were run in triplicate and their relative expression was determined by normalizing the expression of each target to HPRT1 and then comparing this normalized value with the normalized expression in a reference control sample to calculate the fold-change value.
The relative quantification of mRNA expression was performed by normalizing the expression value transformed in relative copy numbers (obtained from Cq values and with efficiency considered).
This was done so as to both verify the efficacy of the QPCR as well as to control for differences in starting RNA concentrations by normalizing the expression values against this gene.
The relative expression level of each FBXW7/hCDC4 isoform was obtained by normalizing the expression of FBXW7/hCDC4 mRNA to GAPDH mRNA expression.
mRNA expression was calculated according to the ΔΔ Ct quantification method and the relative expression level of individual genes was obtained by normalizing the expression to b-ACTIN mRNA expression.
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The relative expression level of Nectin4 in each sample was calculated by normalizing the corresponding expression with G USB expression values.
By further normalizing the expression (10) by (S_{vartheta Omega _{rho }}=2pi rho ^{2}left (1-cos theta _{0}right)), the area of the surface 𝜗 Ω ρ, we obtain the estimation of the mean square magnetic field.
Relative gene expression was determined by normalizing the gene expression of each target gene to GAPDH.
dCtX values were calculated by normalizing the average expression of gene X to the average expression of the endogenous control gene GAPDH.
The relative mtDNA levels were calculated by normalizing the relative expression of mitochondrial Cox1 to the relative expression of the nuclear cyclophilin A. Primer sequences are provided in Supplementary Table S1.
More suggestions(15)
by normalizing the performance
by normalizing the test
by normalizing the stay
by normalizing the alteration
by normalizing the column
by substituting the expression
by normalizing the signal
by normalizing the assay
by normalizing the light
by normalizing the fluorescence
by increasing the expression
by normalizing the completion
by normalizing the slope
by normalizing the follicle
by normalizing the firefly
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