Sentence examples for by normalizing the band from inspiring English sources
Bands were quantitated using reverse image scanning densitometry (Photoshop CS2, Adobe) by normalizing the band intensity of the co-immunoprecipitated protein to the band intensity of the immunoprecipitated protein.
The artificial unit of each protein was calculated by normalizing the band of the protein of interest to the band of loading control, actin.
Changes in protein phosphorylation were calculated by normalizing the band density phospho-Akt to total Akt, and then were presented relative to the untreated group controls.
Immunoreactive bands were quantitatively analyzed in triplicate by normalizing the band intensities to beta-actin on scanned films with Alpha Image software.
The fraction of unwound duplex was obtained by normalizing the band intensities separately for each gel using the parameters from the fit to a one-site binding model, as the change in fluorescence upon unwinding is different under each condition.
B. Calculate the effect of PLX4720, PD184352, or vehicle in the presence or absence of HGF by normalizing the band intensities (after background and loading correction) to the band intensity of the SK-MEL-5 vehicle control condition.
The level of phosphorylation of Serine 1928 on Cav1.2 protein was determined by normalizing the obtained p1928 band to the total CNC1 intensity for the loaded surface fraction.
a From the Ribonuclease Protection Assays (RPAs), mean fold-change was determined by normalizing the optical density band of the LPS-treated sample to the housekeeping gene GAPDH or L-32, then expressing it relative to the optical density of the normalized band from the vehicle-treated sample.
The data were obtained from at least three independent experiments and quantified by densitometry after normalizing the bands to β-actin.
Moreover, to quantitatively evaluate the fluctuations in mobility, we calculated the area of electrophoresed DNA bands by normalizing the area of electrophoresed DNA bands that were digested in buffer containing 10 mM of MnCl2 to 1.0.
By normalizing the radioactivity in each band to the radioactivity of the control DNA oligonucleotide we were able to quantify and compare these data (Fig. 4B; Fig. S1).
