Sentence examples for by normalizing expression levels from inspiring English sources
Samples were run in triplicate and relative expression levels were determined by normalizing expression levels to that of β-actin.
Mathematical modeling was used to identify RNAs stably expressed in all brain regions (serving as potential markers for normalizing expression levels), linked to basic cellular functions.
Genes with stable expression across brain regions and subjects were identified as possible reference RNAs for normalizing expression levels of other RNAs using the following information-theoretic approach.
Samples were run in triplicate, and relative expression levels determined by normalizing expression according to β-actin expression.
Expression levels were estimated by normalizing expression against GAPDH.
The two datasets were combined by normalizing expression of each gene to the highest expressing gene in its set.
qRT-PCR results were analyzed by normalizing expression to the internal control glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
The quantitative polymerase chain reaction detection is the most feasible and cost-effective method to detect miRNA expression; however, next generation sequencing might be used as an alternative method, overtaking the use of normalizing miRNA expression levels.
Activity was calculated by normalizing raw firefly luciferase activity to GFP expression levels measured by western blot.
Differences in transfection efficiency were accounted for by normalizing Luc expression to β-Gal expression (Luc/β-Gal).
