Sentence examples for by normalizing each band from inspiring English sources

Relative density of bands was determined by normalizing each band to lane total protein density determined by a Coomassie blue stain.

The analysis was conducted by normalizing each protein band to its respective loading control.

The fraction of unwound duplex was obtained by normalizing the band intensities separately for each gel using the parameters from the fit to a one-site binding model, as the change in fluorescence upon unwinding is different under each condition.

The artificial unit of each protein was calculated by normalizing the band of the protein of interest to the band of loading control, actin.

Bands were quantitated using reverse image scanning densitometry (Photoshop CS2, Adobe) by normalizing the band intensity of the co-immunoprecipitated protein to the band intensity of the immunoprecipitated protein.

Immunoreactive bands were quantitatively analyzed in triplicate by normalizing the band intensities to beta-actin on scanned films with Alpha Image software.

Changes in protein phosphorylation were calculated by normalizing the band density phospho-Akt to total Akt, and then were presented relative to the untreated group controls.

B. Calculate the effect of PLX4720, PD184352, or vehicle in the presence or absence of HGF by normalizing the band intensities (after background and loading correction) to the band intensity of the SK-MEL-5 vehicle control condition.

Because the immunoreactivity of some antibodies on these samples was somewhat variable, we calculated the averages of immunofluorescent intensities from three blots by normalizing band intensities with those obtained from samples of saline-treated nonTg mice in each blot.

Densitometric analysis was performed by normalizing band density to that for GAPDH.

The level of expression of HDAC1 by normalizing to the band density of nuclear membrane protein lamin A was significantly higher in RA than OA synovial tissue (P = 0.0495) (see Additional file 1).