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The density of cells was determined by normalizing data derived from the barrel cortex in each section to mm.
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Raw data derived by oligo microarray were normalized using lowess algorithm exploiting MIDAW web tool [ 19].
Raw data derived by two-colour cDNA microarray have been normalized using lowess algorithm [ 80] with MIDAW web tool [ 81].
Data derived from the reflective signals were normalized by logarithmic transformation.
For human, COXPRESdb includes coexpression data derived from 79,948 microarrays, whose raw data are extracted from ArrayExpress and normalized by the robust multi-array average method.
The described procedures were applied to both Rank Invariant and Quantile normalized data and the final gene list was obtained by the intersection of the lists, derived by the two normalizations.
To normalize data, we first calculated the mean CT values of all 11 miRNAs to derive a single reference profile as normalization baseline (plotted pink in Figure S1).
The inset shows normalized data.
Relative MMP-1-promoter activation was derived by normalizing the firefly luciferase activity to renilla luciferase activity.
These artifacts cannot be eliminated by globally normalizing the data.
The data was then normalized by a factor derived from the geometric mean of the positive controls.
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