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Changes in abundance of the protein of interest due to treatment were compared by normalising to the untreated cells control.
Relative expression levels of NOB1 mRNA were calculated by normalising to the level of GAPDH expression by using comparative threshold cycle method, in which fold difference is 2 Δct of target gene Δct of reference).
Daily fluctuations of the FACSCalibur flow cytometer were controlled by using Sphero 8 peak Rainbow Calibration Particles (Becton Dickinson & Co) and specific fluorescencent signal were calculated on the live cells by normalising to the appropriate isotype control, as described elsewhere (Pos et al, 2010).
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Polymerase chain reaction reactions were performed in triplicate and relative expression levels of ParvB in normal and tumour tissues were calculated by normalising to GAPDH expression levels by using the comparative CT method.
Relative protein expression levels were quantified by normalising to actin expression levels.
TCa was corrected for albumin (corr-Ca) by normalising to an albumin concentration of 36 g/l using a correction factor of 0.016 mmol TCa/g albumin.
MRP-1 protein expression, detected by western blot, was normalised to the loading control and relative to the scrambled siRNA control.
In an ergodic model, the activities of signalling proteins, usually given by their phosphorylated forms normalised to the maximal signal, could be correlated with the steady-state probability distribution on the state space of the PBN model.
Samples were assayed using triplicate wells for each gene of interest and copy numbers were estimated by relative quantitation (RQ) normalised to the known copy number of the reference sequence using the comparative Ct (ΔΔCt) method.
All accumulated substances are quantified by gas chromatography, summarised and normalised to the reference compound 2,3-dimethylnaphthalene (log KOW = 4.4).
The band of protein expression was quantified by Image J software and normalised to the β-actin expression level.
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