As an intermediate neurofilament component, the normal staining pattern of INA by an anti-INA monoclonal Ab (mAb) was characterized within the cytoplasm of the cells and also in the cell processes (Figure 6B, also seen in Figure 2B C).
Although myocardial development appeared normal by staining of the sarcomeric protein MF20, disruption of AV canal cushion development, and possibly decreased myocardial function, was common in CsA-treated and 2APB-treated zebrafish (Fig. 5A and B).
Normal staining was defined as ≥70% membranous staining of the cancer cells [ 29].
Absence of nuclear staining in tumor cells and normal staining in the surrounding normal tissue were considered as MMR-deficient.
IHC of E-cadherin also showed positive staining (normal membrane staining of tumor cells).
The CD markers constitute the most widely studied hematopoietic markers, with known protein expression patterns confirmed by antibody staining of normal and malignant hematopoietic cellular elements.
To test this possibility, we investigated latexin expression in 41 gastric carcinomas and paired corresponding adjacent normal tissues by immunohistochemical staining of tissue sections.
We, therefore, co-investigated the nuclear expression of CSPP1 and cytokeratin-8 (Taylor-Papadimitriou et al, 1989), or smooth muscle actin (Deugnier et al, 1995) respectively, by immunofluorescence staining of normal breast epithelium.
*Not Determined In order to investigate Cav-1 expression in different stages of hepatocellular carcinogenesis, we analyzed its expression profile by immunoperoxidase staining of normal liver (n = 20), cirrhosis (n = 29), and HCC (n = 95) tissue samples.
The specifity of the antibody for δ-toxin is demonstrated by lack of staining of normal human keratinocytes in the absence of δ-toxin but positive staining in the presence of δ-toxin (Figure 1c).
