Sentence examples for by nonlinear regression fitting from inspiring English sources

The final temperature of 260°C was held for 1.5 min. The total run time for the analysis was 25.0 min. Ymax was defined by nonlinear regression fitting the extraction duration plot to Y = Ymax*X/(K+X).

For each compound, parameters for the model were derived by nonlinear regression

The product formation rates (as a function of dNTP concentration) were plotted to estimate the kinetic parameters Km and kcat by nonlinear regression fitting to the Michaelis Menten equation using Graph Pad Prism 5.0 software (GraphPad, San Diego, CA).

For statistical analysis, best-fit estimates of the IC50s and their 95% confidence intervals (CIs) were obtained by nonlinear regression fitting of the sigmoidal dose-response curve for each drug across all experiments after normalization using control well fluorescent signals.

All enzymatic activities were determined in triplicate, and the maximum velocity (Vmax), Michaelis constant (KM), and catalytic constant (kcat) were calculated by nonlinear regression fitting of the data to the semi-logarithmic form of the Hill equation using the SigrafW software [ 66].

Sigmoidal dose-response curves were generated by nonlinear regression fitted to Hill equation to compute maximum effect (Emax), slope (N), and the effective dose reaching 50% of the Emax (ED50).

The concentration of drug that reduced cell proliferation by 50% (IC50) vs controls was calculated by nonlinear regression fit of the mean values of the data obtained in triplicate experiments (at least nine wells for each concentration).

Then, the data were analyzed by nonlinear regression fit of association kinetics with multiple concentrations in Graph Pad Prism 6. A431 cells (ATCC) were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FisherBrand).

The fluorescence intensity was measured at the excitation of 636 nm and emission of 664 nm, and then the results were analyzed by nonlinear regression fit of one-site ligand depletion model in GraphPad Prism 6. Association rate was measured by adding fluorogen with various concentrations to constant probe.

Results of seasonal variations were tested with a commercial statistical software (Statistica version 7.1; Statsoft, Tulsa, OK, USA), by using nonlinear regression fitting a sine function.We considered p values <0.05 statistically significant.

Thus, data were analyzed by nonlinear regression curve fitting (one site fits Ki), using the concentration of NPN (typically 5000 nM as HotNM) and Kd for NPN in nM (HotKdNM).