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Michaelis-Menten constant (Km) and catalytic constant (kcat) were obtained by nonlinear regression applying the Michaelis-Menten model in the Anemona program (Hernández and Ruiz 1998).
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Four kinetic models, Thomas, Adams-Bohart, Yoon-Nelson, and Clark, were applied to experimental data to predict the breakthrough curves by nonlinear regression and to determine the characteristic parameters of the column that are useful for process design [25].
The isotherm data can well be fitted with four adsorption isotherm models by nonlinear regression.
Kinetics was established by nonlinear regression between Y TP and t.
The IC50 values were calculated by nonlinear regression.
Ki values were determined by nonlinear regression analysis using PRISM GraphPad Softwaree).
Equation 1 was deduced for the two-state model by nonlinear regression analysis.
IC50 values were determined by nonlinear regression sigmoidal dose-response analysis with variable slopes.
The data were analyzed by nonlinear regression using GraphPad Prizm 3.0.
Data were analyzed by nonlinear regression using Prism software version 4 (GraphPad).
Data were plotted by nonlinear regression analysis (curve fit) using Graphs Pad Prism software (version 5).
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