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The content of NH2 groups was determined by ninhydrin method using an amine containing monomer (N- 3-aminopropyl) methacrylamide) as the calibratioN- 3-aminopropyled from [45]].
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Total amino acid content was measured by manual ninhydrin method using leucine as standard (Cheng et al. 2010).
Amino acids analyses were made by the ninhydrin method using an automatic analyzer Beckman, model System 6300.
The –COOH density on/in the PMAA grafted PLLA membrane/scaffold was measured by colorimetric method and the collagen content on/in the collagen immobilized PLLA membrane/scaffold was measured by ninhydrin method.
The results of ATR FTIR measurement demonstrated that PPC was aminolyzed by polyethylenimine (PEI) at specific reaction conditions and the degree of aminolyzation was quantitatively determined by ninhydrin method.
Figure 5 Standard plot of pregabalin by ninhydrin method.
Amino acids were defined by ninhydrin method [17].
The concentration of peptides on the scaffolds was measured at 106±4 μmol/cm2 using the ninhydrin method and 92±9 μmol/cm2 using the BCA protein assay.
CUPPING Tasting method used by coffee professionals.
Amino acid analysis was carried out using Ninhydrin method (Sykam Amino Acid Analyzer S433; Sykam, Eresing, Germany).
Total amino acids were measured by the ninhydrin method [ 86].
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