Sentence examples for by negative staining using from inspiring English sources

Bands corresponding to MMP2/9 activity were visualized by negative staining using Coomassie brilliant blue.

The sample was visualised by negative staining using 1% (w/w) uranyl acetate and viewed on a JEOL 1220 transmission electron microscope fitted with a SIS Megaview III digital camera system.

The sample was then subjected to gradient fixation using GraFix (Kastner et al., 2008), grids were prepared by negative staining using uranyl acetate, and images were acquired in a Tecnai F20 electron microscope under low-dose conditions.

Bands corresponding to the activity were visualized by negative staining using Coomassie Brilliant blue R-250 (Bio-Rad Laboratories, Richmond, CA, USA) and molecular weights were estimated by reference to prestained SDS-PAGE markers.

Tld (10 µl at 34 μg/ml) was analysed by negative stain using 2% phosphotungstic acid at pH7.4.

The fraction showing the strongest signal in dot-blot was prepared by sandwich negative staining using 2% uranyl acetate [20].

The specificity of the anti-NXF2 monoclonal antibodies was confirmed by ELISA and by positive immunohistochemical staining of spermatogonia in testis and negative staining using a panel of normal adult tissues (data not shown).

The standard two-step drop method, i.e., adsorption followed by negative staining, is used for preparation.

The specificity of the immunostaining was confirmed by negative control staining using mouse nonimmune immunoglobulin G instead of the primary antibody.

By taking advantage of the preferred orientation induced by negative staining, image alignment and classification could be used to distinguish differences in the molecular conformation without having to simultaneously address differences in orientation.

To examine VLP, pooled faster sedimenting fractions (5 14) from plant samples were immobilized using rabbit anti-NaVCP antibody followed by negative staining and visualized by electron microscopy.