Sentence examples for by negative sorting using from inspiring English sources

Briefly, CD14+ monocytes were purified from PBMC isolated on Pancoll (Pan Biotech, Aidenbach, Germany) by negative sorting using Dynabeads (Dynabeads Untouched Human Monocytes, Invitrogen, Oslo, Norway).

Endothelial cells were cultured and purified over a 2- to 3-week period by a series of magnetic immunosortings with Dynabeads (Invitrogen), including a single negative sort using antibodies to the Fc III/II receptor (Abd Serotec) to remove macrophages, followed by at least two positive sorts using antibodies to ICAM-2 (Abd Serotec) to enrich for the endothelial cell population.

B cells were then selected by negative sorting by using EasySep Human B Cell Enrichment Kit Stemcell Technologies Grenoblee, France).

T cells were purified from peripheral blood mononuclear cells by negative sorting with magnetic beads (Pan T-cell Kit, Milteniy Biotec, Auburn, CA).

Experiments were carried out on spleen, lymph nodes and bone marrow suspensions, or splenic B-cells negatively purified by immunomagnetic sorting using the Dynabeads Mouse CD43 Negative Isolation Kit (Invitrogen) (>85% purity).

CD4 and CD8 T cells were then isolated from the CD14 and CD19 negative fractions, respectively, by magnetic cell sorting using CD4 and CD8 microbeads as described by the manufacturer.

Propagated cells negative for p75NGFR Schwann cell surface marker were separated by magnetic cell sorting system followed by FACS sorting using anti-p75 antibody (AB1554, Millipore, USA).

Neutrophils were then isolated by magnetic cell sorting using CD16 microbeads as described by the manufacturer.

To determine the frequencies of ex-vivo CSR, resting B-cells were isolated from dissociated spleen through magnetic sorting by negative selection using an antibody cocktail against CD43 (Ly-48), CD4 (L3T4), and Ter-119 (Miltenyi Biotech).

Active sorting using acoustic forces can be improved by combining it with passive sorting methods.

The EACs were sorted by negative selection using anti-CD3 and anti-CD16 antibody coated micro-beads (Milteny Biotech).