Sentence examples for by negative selection with from inspiring English sources

Human CD4+CD45RA+ naïve T cells were purified from PBMC by negative selection with magnetic beads (Miltenyi Biotech, Germany).

T cell-depleted splenocytes (Tds) were obtained by negative selection with murine anti-CD4 and anti-CD8 microbeads (Miltenyi Biotec).

Gld DN and CD4 T cells (>95% pure) were isolated by negative selection with Dynal beads as described above.

T cells were incubated with anti-CD4 microbeads and CD8 T cells purified by negative selection with MACS separation columns (Miltenyi Biotech).

Purified CD4+ T cells were obtained by negative selection with the CD4 T-cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany).

NK cells were isolated from mouse spleen by negative selection with an NK isolation kit (Miltenyi Biotec, Auburn, CA), and were >90% CD3−, NK1.1+, CD49b+ (DX5+), and CD16+.

The rosette-positive cells were further purified by negative selection panning with mAbs to CD8, CD16, CD19, HLA-DR, and CD45RO as described previously [29].

It should be noted that in the MGM the frequency of every facilitating gene (FG; a gene that increases the risk and the severity of SCZ in the presence of a pathogenic mitochondrial genome) on any chromosome may diminish in the predisposed matrilineal pedigrees by negative selection; thereby, negatively associating with SCZ.

Murine B cells were isolated by negative selection (Miltenyi, Germany), with a routine purity of >95%, and stimulated with lipopolysaccharide 10 μg/mL (0111 B4) (Sigma, UK) and IL-4 (10 ng/mL).

For DC isolation from LN (n = 30 35 mice/sorting), CD11c+ cells were enriched by negative selection after incubation with a mix of specific mAbs (FTIC-conjugated mAbs against CD19, DX5 and TCRβ), and anti-FITC magnetic microbeads using MACS cell sorter (Miltenyi Biotec) in accordance with the manufacturer's instruction.

CD4+CD45RA cells were isolated from the CD4+CD25− cells by negative selection and activated with anti-human CD3/CD28 beads 1∶10 (one bead to 10 cells) in AIM-V serum-free medium containing Hepes buffer (10 mM), sodium pyruvate (1 mM), glutamine, non-essential amino acids and penicillin and streptomycin.