Sentence examples for by negative selection using from inspiring English sources

Negatively selected PBLs were used to isolate naïve CD4+ T cells by negative selection using the Naïve CD4 T cell isolation kit II (Miltenyi Biotec).

Naïve (CD19 + CD27-) B cells were selected from total CD19+ B cells by negative selection using CD27+ microbeads (Miltenyi Biotec).

To isolate memory T cells, naive CD4 + T lymphocytes were removed by negative selection using anti-CD45RA (eBioscience) and depleted by incubation with Dynabeads Pan Mouse IgG (Invitrogen).

After hemolysis, the CD4+ T cells were separated from the splenocytes by negative selection using a CD4+ T-cell isolation kit (Miltenyi Biotec) and passing the cells over a MACS LS column (Miltenyi Biotec).

Mo were isolated by negative selection using immunobeads (Miltenyi) [17], [25] or by FACS sorting.

CD4+CD25− cells were purified by negative selection using magnetic beads.

Total CD4 T cells were isolated from whole splenocytes by negative selection using magnetic beads (Miltenyi).

CD4+ T-cells were prepared by negative selection using the MACS separation system (Miltenyi Biotech).

Subsequently, CD8 T cells were purified from the cultures by negative selection using magnetic beads.

Pure T cells (>95%) were obtained by negative selection using magnetic cell sorting.

CD8+ cells were isolated from stimulation cultures by negative selection using a CD8+ T cell isolation kit (Miltenyi Biotec).