Sentence examples for by negative selection over from inspiring English sources

The cells migrating between the densities of 35% and 50% Percoll were collected, and then they were subjected to immunopurification by negative selection over columns consisting of magnetic microbeads coupled to mouse anti-human HLA class I antibody at a concentration of 40 µg/ml (clone W6/32; eBioscience, San Diego, CA, USA).

Since the synthesis and secretion of venom proteins is energetically expensive [ 65- 67], mutations that disrupt the structure/function of proteins are filtered out of the population by negative selection over time, favoring the conservation of catalytic and structurally important residues.

CD4+ and CD45RO− T cells were isolated by negative selection (Miltenyi Biotec) and were over 90% pure.

B cells from six individual donors were isolated from human buffy coats (National Blood Service, Watford, UK) by centrifugation over a density gradient followed by negative selection using magnetic beads.

Microglia isolated by positive selection were >99% pure and free from astrocytes, while astrocytes collected by negative selection were 95 97% pure and completely free from microglia.

CD4+CD25− cells were purified by negative selection using magnetic beads.

First, untouched CD4+ T cells were isolated by negative selection.

Mo were isolated by negative selection using immunobeads (Miltenyi) [17], [25] or by FACS sorting.

Single positive CD4 cells were isolated by negative selection using the CD4 negative isolation kit II (Miltenyi Biotec).

Alternatively, CD4+ T cells were isolated from cell suspension by negative selection and automacs sorting (Miltenyi).

T cells were prepared by negative selection as described previously to a purity of >95% [8].