Sentence examples for by negative selection on from inspiring English sources
First, a phage-displayed random cyclic peptide library was depleted of phage binding to normal cells by negative selection on either myoblasts (panning I) or fibroblasts (panning II).
Purified T cells were obtained by negative selection on AutoMACS cell separator using the Pan T-cell isolation kit (Miltenyi Biotech) and placed in RPMI 1640 (Biofluids) supplemented with 5% dialyzed FCS (heat inactivated) and 100 U/ml penicillin, 100 mg/ml streptomycin, 1 mM sodium pyruvate, 1 mM HEPES, and non-essential amino acids (RP5).
Total CD4+ T cells were isolated by negative selection on a Robosep (Stemcell–Human CD4+ T cell enrichment kit).
Non-adherent monocytes purified from buffy coats by negative selection on magnetic beads had widely varying copy numbers of mRNAs for MMPs and TIMPs.
Primary T-cells were isolated from 3-4 mouspleensens by negative selection on magnetic bids using the Pan T cell Isolation Kit II (Miltenyi Biotech), see Grum-Schwensen et al. for details [ 13].
Individual LEU2 transformantsmants were patched to SD-LEU-TRP plates and cured of the wild-type copy of RPB2 by negative selection on media containing 5-fluoroorotic acid (Boeke et al. 1984).
For transfection experiments, OPCs were purified from P7 Sprague-Dawley rat brains as described previously [25] and purified by a negative selection on Ran-2 and GalC-coated immunopanning plates followed by a positive selection on O4-antibody coated immunopanning plates.
Eosinophils were isolated as described [61] on Percoll followed by negative selection using anti-CD16-coated microbeads.
On day 3, CD8+ T lymphocytes were isolated by negative selection using CD8a+ T-cell isolation kit (Milternyi Biotec) according to the manufacturer's instructions.
