Sentence examples for by negative selection for from inspiring English sources

As there is no one DC specific marker, as has been described previously [35], DCs were gated by negative selection for CD3, CD14, CD19, CD56 and positive selection for HLA-DR as shown in Figure 6.

Human placental mesenchyme for culture was isolated by negative selection (for details see the Supplemental Information).

To minimize granulocytes proportions in the BAL, macrophages were purified by negative selection for Ly6G expression (a marker exclusively expressed on neutrophils and eosinophils), and pools with 95.86% ± 0.25% (SEM) purity were obtained (Additional file 1: Figure S3 and Additional file 3: Table S2).

CD25− fraction (effector population) was isolated by negative selection from the remaining CD4+CD45RO+ fraction with the addition of 106/50µl cells of CD25+ positive selection Dynabeads at room temperature for 40 minutes.

The estimation of ancestral gene expression levels is not trivial, especially for traits that are heavily affected by negative selection and for which the ancestral values might therefore frequently fall outside the range observed in extant species.

This high LD might be related to LD inflation by negative selection, as indicated for SNP SNP LD (38, 39).

For adoptive transfer-induced experimental colitis, CD4 T cells were enriched from total splenocytes by MACS negative selection for B220, CD11b, CD49b, CD8, and Ter119.

This paradoxical result is probably explained by a negative selection for surgery due to apprehension for increased complication rates and poor outcomes in obese subjects.

In brief, CD4+ T cells were enriched by negative selection and then went through positive selection for CD25+ T cells.

In some experiments, T cells purified by negative selection as above were cultured for 6 hours in wells precoated with antibodies to CD3 (UCHT1) and CD28 (clone2 cloneacheacoatedted at 5 μg/ml in the presence or absence of cyclohexamide (10 μg/ml).

Many of these SNPs may eventually be eliminated by negative selection but, as already suggested for intracellular bacteria [ 46– 49], the high levels of chaperonin expression that we observed may also suppress the phenotypic effects of these deleterious mutations in Microsporidia [ 41].