Sentence examples for by negative selection by from inspiring English sources

DN T cells were isolated by negative selection by using Dynabeads (Dynal®, Lake Success, NY) as previously described [42].

Unlabelled T lymphocytes were then enriched by negative selection by placing them within the magnetic field of the BD IMagnet™ as per the manufacturer's instructions.

Monocytes were then purified from PBMCs by negative selection by using a magnetic-activated cell sorting (MACS) monocyte isolation kit (Miltenyi Biotech, Auburn, CA, USA).

A CD4+ T-cell population was prepared by negative selection by MACS with anti-CD19 mAb, anti-CD11c mAb, and anti-CD8a mAb (BD PharMingen).

The CD4+ cells were collected 8 days after immunization (with either OVA, CII, or A12 emulsified in CFA) by negative selection by using ferromagnetic beads, according to the manufacturer's protocol (Miltinyi Biotech).

To isolate memory T cells, naive CD4 + T lymphocytes were removed by negative selection using anti-CD45RA (eBioscience) and depleted by incubation with Dynabeads Pan Mouse IgG (Invitrogen).

Hensel, M. et al. Simultaneous identification of bacterial virulence genes by negative selection.

Where indicated, CD8 T-cells were isolated by negative selection (Stemcell).

Baddoo, M. et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection.

In contrast, 3A9 T cells (specific for hen egg lysozyme(46-61)/I-A k)) are completely eliminated by negative selection.

All samples were acquired with a FACScalibur or FACSCanto (Becton Dickinson Immunocytometry Sistems) and analyzed with FlowJo software (Treestar Inc .. B cells were isolated from BM by negative selection with immunomagnetic beads followed by cells sorting.