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Naïve (CD19 + CD27-) B cells were selected from total CD19+ B cells by negative selection using CD27+ microbeads (Miltenyi Biotec).
Hensel, M. et al. Simultaneous identification of bacterial virulence genes by negative selection.
Where indicated, CD8 T-cells were isolated by negative selection (Stemcell).
Baddoo, M. et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection.
To isolate memory T cells, naive CD4 + T lymphocytes were removed by negative selection using anti-CD45RA (eBioscience) and depleted by incubation with Dynabeads Pan Mouse IgG (Invitrogen).
Bedel, R. et al. Effective functional maturation of invariant natural killer T cells is constrained by negative selection and T-cell antigen receptor affinity.
In contrast, 3A9 T cells (specific for hen egg lysozyme(46-61)/I-A k)) are completely eliminated by negative selection.
This study evaluated the immunologic effects of thymoglobulin in modulating human CD4+ cells.Human CD4+ cells were purified from peripheral blood mononuclear cells by negative selection method.
Human CD4+CD45RA+ naïve T cells were purified from PBMC by negative selection with magnetic beads (Miltenyi Biotech, Germany).
First, untouched CD4+ T cells were isolated by negative selection.
Mo were isolated by negative selection using immunobeads (Miltenyi) [17], [25] or by FACS sorting.
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