Sentence examples for by negative purification using from inspiring English sources
For NK cell enrichment PBMCs were isolated from de-identified healthy donor buffy coats using ficoll density centrifugation with Bicoll Separating Solution (Biochrom, Berlin, Germany) and NK cells were obtained by negative purification using the NK Cell Isolation Kit II (Miltenyi Biotech, Bergisch Gladbach, Germany) according to the manufacturer's recommendation.
Thus, the purified recombinant HP-NAP obtained by negative purification using DEAE Sephadex anion-exchange resin retained its oligomeric status with α-helical structure.
Our previous study has shown that recombinant HP-NAP expressed in B. subtilis can be obtained in high purity by negative purification through the collection of the unbound fraction using DEAE Sephadex anion-exchange resin at pH 7.5 and 8.0 [ 26].
Splenocytes were harvested, and CD8+ T cells were isolated by magnetic purification using a Dynal mouse CD8 negative isolation kit (Oslo, Norway) according to the manufacturer's instructions.
The next day, DNA was purified from the reverse-crosslinked chromatin by proteinase and RNase digestion followed by purification using Qiagen DNA purification columns.
RNA was isolated by phenol-chloroform extraction, followed by additional purification using RNEasy mini spin columns (Qiagen).
The PCR products were purified using a QIAquick PCR purification kit (QIAGEN), a High Pure Kit (Hoffman LaRoche), or by enzymatic purification using EXO-SAP-IT (USB Europe).
Chloroform was added for phase separation followed by RNA purification using the RNeasy Mini Kit (Qiagen).
The peptide-specific antibodies were purified by affinity purification using peptide conjugated to AffiGel-10 (Biorad).
We extracted RNA from tissue using RNAiso Plus (Takara Bio), followed by purification using RNeasy MinElute (Qiagen).
Total RNA was isolated using a Trizol® extraction followed by purification using an affinity column (www.affymetrix.com).
