However, notwithstanding the greatly enhanced ISGylation capacity by mutation of the single Asn 89 residue in HuISG15 to an Asp residue, the overall ISGylation pattern in human HekT cells was still intermediary compared to AgmISG15.
In maize, the transition from the mitotic to meiotic cell cycle can be abolished by mutation of a single gene, ameiotic1 (Golubovskaya et al. 1993, 1997).
A less promiscuous NLS that is completely deactivated by mutation of one single amino acid combined with the photocaged amino acid could yield tight optical control of nuclear entry and provide the basis for a wide range of applications.
Overall, these experiments have showed that, in the GC1 cells, the loss of CCDC6 or its functional impairment by mutation of a single residue limited the amount of S139_pH2AX that then influenced the sensing of DNA damage induced by H2O2, relative to the control cells.
In spite of this, an 8 10% frequency of effect is at least a thousand times greater than that expected, were EC adhesion be caused by mutation of a single gene (0.01%) induced by a similar X-ray dose (Ellender et al., 2005).
The inhibition of differentiation and persistence of proliferation in cell transformation is probably not only caused by the mutation of single genes.
This scenario of duplication and restriction, rather than duplication and recruitment (fig. 1) is more parsimonious as it requires only the loss of transcription factor binding sites, which may occur by random mutation of single base pairs or larger insertions or deletions (indels) that may delete or disrupt the existing transcriptional regulatory sequences.
For 3TC an additional explanation is that this drug is highly influenced by mutations of a single residue, M184, to V or I.
This finding can be accounted for by independent mutations or by mutations of a single locus with positive pleiotropic effects on sexual and asexual reproduction.
