To test this notion, supernatants were collected and analyzed for the production of a panel of 22 cytokines by multiplex analysis (complete cytokine results are contained in Table S1).
Supernatants harvested from cultures with cells isolated from WT mice were analyzed for IL-10 and interferon-gamma (IFN-γ) expression simultaneously by multiplex analysis using the Luminex 100 system (Becton Dickinson, Mountain View, CA).
Analyses of cytokine, chemokine, and growth factor production were extended by multiplex analysis (Figure 6).
Analytes were measured by multiplex analysis (Bio-Plex Laboratories, HerCAles, CA, USA) using 96-well multiplex kits (Millipore, Billerica, MA, USA).
Multiplex analysis kits for leptin, resistin, and total adiponectin were obtained from Millipore, Hamburg, Germany.
In a recent study by Ingvarsson et al, 2007, sandwich antibody microarrays were used for multiplex analysis of eight complement proteins (including C3) in unfractioned serum and plasma [23], showing promising results for protein profiling in complex samples.
Additional benefits exhibited by the HyBeacon technology derive from their simple mode of action, ease of design, relatively inexpensive synthesis and potential for multiplex analysis.
PCR conditions were then optimized for multiplex analysis of the targets.
were selected for multiplex analysis.
In this application, HCR probes were labeled with different fluorophores for multiplex analysis.
