Sentence examples for by multiple washes with from inspiring English sources

Unbound protein was removed by multiple washes with binding buffer and bound protein was eluted in 100 µl elution buffer (10 mM glutathione, 50 mM Trizma-HCl, pH 7.4) at room temperature for 30 minutes.

Following 2 hr infection, the cells were removed of remaining viruses by multiple washes with fresh medium.

The maleimide thiol reaction was allowed to proceed for at least 10 h at 25 °C, followed by multiple washes with PBS, to remove unbound antibody.

Mineralization of calcium deposits was assessed by Alizarin Red S staining (40 mM, pH 4.2) (LabChem, Pittsburgh, PA, USA) for 10 min at room temperature, followed by multiple washes with distilled water to remove background staining [ 26].

Wash the affected region of hair two to three times with the dish soap, followed by multiple washes with shampoo.

Next, this template was removed from the former film by multiple washing with toluene, ethanol, and then water to allow determination of pyrene.

Excess paraffin was removed by multiple washes in xlyene.

Post-infection samples were washed extensively and partially attached and non-ingested beads/bacteria are removed by trypsin treatment followed by multiple washes.

The troughs were filled with lipids by immersing the test device in a solution of liposomes, 1,2-dihexadecanoyl sn-glycero-3-phosphocholine [PC 16:0/16:0)], and cholesterol (Avanti Polar Lipids, USA), followed by multiple washes to remove lipids deposited on the PEG.

Fusion was initiated by addition of 40% PEG (1500) in PBS for 50 sec, followed by multiple washing steps with PBS and further incubation in medium at 37°C for the indicated time periods.

After 4 h of incubation at 37°C in modified Medium199, non-phagocytosed parasites were removed by multiple washing steps with PBS and incubation was continued for another 20 or 44 h in IMDM at 37°C and 9% CO2.