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For the proteins, the multiple column charts represent the presence of post-translational modification (proteins that were represented by multiple spots) on pathways figures.
According to our experience, Stouffer's method is the only available method yielding plausible results if genes are resampled by multiple spots on a chip and if these might exhibit inconsistent fold-changes and significantly varying expression levels.
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A second analysis, filtering on confidence, was possible because genes were represented on the chip by multiple spots.
Individual proteins were reflected by multiple spots most likely due to posttranslational modification leading to shifts in the 2-DE.
On virtually all current microarray platforms, a subset of the genes are represented by multiple spots or probe sets.
Moreover, among the 35 differentially expressed proteins, 10 proteins were present as multiple spots on the 2-DE gels, with one spot representing an isoform (Figure 4A).
However, it is well known that Hsp27 is extensively modified by phosphorylation leading to reciprocal abundance changes of multiple spots on 2D gels [ 36, 37].
Compositional analysis was carried out at multiple spots on the gold-colored layer by EDX.
A single band by WB may have dozens of different protein species when analyzed by mass-spectrometry (Thiede et al. 2013 ) or multiple spots on 2D gels.
Doctors inject it into multiple spots on the neck, relieving sometimes disabling pain for weeks or months.
Importantly, the same protein may be present in multiple spots on a gel.
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