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Sequences were aligned by multiple sequence alignment technique using CLUSTAL W [ 13] and a phylogenetic tree constructed by the neighbor joining method [ 14] using MEGA version 5 [ 15].
This is followed by multiple sequence alignment.
The corresponding residues for the other 10 sequences were identified by multiple sequence alignment [40].
Comparison by multiple sequence alignment generated a dendrogram, revealing the phylogenetic relationships of CrGSTs with other well-characterized GST classes.
The primers were designed, by multiple sequence alignment of variable regions, specific for cold-adapted donor virus HTCA-A101, as compared to other influenza A viruses.
Obtained nucleotide sequences were compared with reference japonica genome by multiple sequence alignment and all the nucleotide sequences were translated into amino acid sequences.
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The marine fish microsomal epoxide hydrolase (mEH) of Mugil cephalus was engineered to enhance the enantioselective hydrolytic activity by multiple sequence alignment-inspired mutagenesis.
As affirmed by multiple sequence alignments, most of the functionally important residues were well conserved in RfCat.
Substitutions F26V and I83R predicted by multiple sequence alignments were introduced into mutant 1X-1, and the t1/2, 50 °C and T5015 of mutant 3X (Q122R, F26V and I83R) increased to 170.8 min and 55.4 °C.
This review brings together the available knowledge of the RGI modifying enzymes and provides a detailed overview of biocatalytic reaction characteristics, classification, structure-function traits, and analyses the protein properties of these enzymes by multiple sequence alignments in neighbour-joining phylogenetic trees.
These previous works were all based on limited sets of sequences (less than 350 proteins) compared by multiple sequence alignments.
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