Sentence examples for by multiple restriction analysis from inspiring English sources

The plasmid constructs were verified by multiple restriction analysis.

The obtained plasmid constructs were verified by multiple restriction analysis.

The plasmid constructs were verified by multiple restriction analysis For chromosomal insertions, E. coli S17-1 λ pir [ 69] was transformed with the appropriate suicide plasmid by CaCl2 method [ 26] to obtain the donor strains.

The plasmid constructs were verified by multiple restriction analysis For the construction of Gram-positive metal sensor and control strains, the plasmids p cadCPcadAlux and p602/22lux were inserted into Staphylococcus aureus RN4220 [ 72] and Bacillus subtilis BR151 [ 73] or BR151 pBL1) [ 38] by electroporation [ 74, 75].

Molecular diagnostic criteria for FSHD are complex and involve analysis of high molecular weight (HMW) genomic DNA isolated from lymphocytes, followed by multiple restriction digestions, pulse-field gel electrophoresis (PFGE), and Southern blotting.

Multiple BAC clones were anchored to the genetically mapped ones, either by inferring overlap (established by restriction analysis and confirmed by PCR), or by BAC-to-BAC ligation through PCR (using STS markers derived from BAC-end sequences), or in some cases, by alignment of identical end sequences.

Identification of leptin gene A19G polymorphism was done by PCR followed by restriction analysis [ 16].

Adiponectin gene T45G polymorphism was identified by PCR followed by restriction analysis [ 19].

Clones were confirmed by restriction analysis and by DNA sequencing of both strands.

Cloned genes were confirmed by restriction analysis and by DNA sequencing of both strands.

Verification of all plasmids was performed by restriction analysis and by DNA-sequencing.