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A degenerate primer pair specific to catA sequences was designed by multiple alignment of known sequences coding a key intermediate of the β-ketoadiapate pathway degrading catechol, namely catechol 1,2-dioxygenase.
Degenerate PCR primers were designed by multiple alignment of the protein sequences of known structural genes encoding the catalytic subunits of NiFe-hydrogenases obtained from Swiss-Prot Protein Sequence Database through CLUSTAL-W software and compared for conserved sequence motifs.
Two SNPs (Single Nucleotide Polymorphisms) were identified within exon 8 by multiple alignment of DNA sequences obtained from 24 bulls: a transition G/A (SNP 1181) and a transversion A/T (SNP 1299).
60% of the A. nidulans genome was covered by multiple alignment of 2 more genomes.
40% of the A. nidulans genome was covered by multiple alignment of all 7 genomes.
The algorithm is based on a phylogenetic hidden Markov model that uses best-in-genome pairwise alignment for each species (based on BLASTZ), followed by multiple alignment of the twenty eight genomes.
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The overall consensus binding motif was obtained by multiple alignments of the significant binding sequences with ClustalW, and it was visualized with SeqLogo program.
We therefore probed relationships among 3' end sequences of mature C. elegans miRNAs by multiple alignments of the 3' sequence of each miRNA against 3' sequences of all the remaining miRNAs.
Conserved elements, as defined by multiple alignments of 44 vertebrate species, and repetitive element data sets were also downloaded from the UCSC Genome Browser.
The extent of genetic diversity in the genus Giardia was assessed by multiple alignments of the TPI nucleotide sequences followed by estimates of genetic distances (Table 2).
In addition, 18 SNPs differed between the A and C genomes, as shown by multiple alignments of 16 FAE1 sequences from the three Brassica species (Table 4).
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